Plant Biotechnology

The Creation Of Transgenic Plants

Transgenic Plant Biotechnology

Endless Possibilities

Disease Resistance: Practicalities

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Why can’t famers buy this product?

Issues

Requirements -1

Agrobacterium

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Biolistics

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Requirements-2

Requirements-3

Problems-1

Problems-2

Problems-3

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Problems-5

Problems-6

Approaches to Genetic Engineering for Disease Resistance

Possible Approaches

Using R-genes continued

Using R-genes- continued

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Using R-genes- Custom Design

Directed molecular evolution- Gene Shuffling

Gene shuffling has been used to identify recognition determinants in R-genes

Using R-genes: finding novel R-genes from other species

More than a third elicited a reaction in at least one genotype

Can express effectors in plant pathogens infecting the target host

Effectors and R Genes Can Work Across Species

So what?

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This is essentially what was done in:

Effectors and R Genes Can Work Across Species

Gene Silencing -RNAi

RNAi

Genetic Engineering Success Stories

RNAi

Does RNAi have broader applications for Transgenic Disease Resistance?

Insects

Nematodes

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Other Approaches to Engineering Disease Resistance in Plants

Existing Defence Response

There are numerous examples of both these approaches ‘working’ in the literature

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Why are there no commercial transgenic products for fungal or bacterial resistance?

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Personal View

So what’s the Point?

Banana

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Genetic Transformation- the best method for Banana Improvement