Plant Genomes Can Be Larger Than The Human Genome

A small Portion of the Genome Comprises Genes

PowerPoint Presentation

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BAC Fingerprinting

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What are the advantages of obtaining a complete inventory of plant coding sequence?

Detailed questions that may be approached through a genomics approach include:

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Arabidopsis contains 41 described gene families

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The tomato and potato genomes are very similar

Tomato-pepper co-linearity is dispersed

1-2% of plant genomes are composed of R gene clusters

Sequencing the Maize (B73) Genome

The Plan

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Minimum Tile Path Pipeline

Minimum Tile Path Pipeline Usage

Maize Production Sequencing

Maize BAC shotgun

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www.maizesequence.org

MapView

CytoView

ContigView

SyntenyView

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“Methyl-filtering” Approach for Genome Sequencing

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Maize Missouri 17 “chromosome 10” project update

Aims: “Plan A”

Challenges

Project goals for researchers and breeders

Milestones

Milestones

Even in expert hands, purity of chromosome prep is 85-90%

Alignment of Mo17 “gene space” with B73 allele ~97% identity

JGI Sorghum update

Early peek at Sorghum-rice comparison shows syntenic segments

Maize divergences (transversions)