Why genomics?

Structural

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Developing World Wants More Protein

Therapeutic Proteins

What improvements should be targeted in crop improvement research?

Improvements Needed

Improvements Needed

Improvements Needed

Biotech Sales

Where will we find genes that confer hardiness?

Weeds and Primitive Crops Have Advantageous Traits

How to Improve Crops…

Why sequence plant genomes?

Detailed questions that may be approached through a genomics approach include:

What are the advantages of obtaining a complete inventory of plant coding sequences?

Plant Genomes Can Be Larger Than The Human Genome

A small Portion of the Genome Comprises Genes

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ESTs: Taking Advantage of the Cell to Sequence the Genes

The Problem: ESTs Miss Rarely Expressed Genes

How did ESTs do?

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A whole-genome clone-based map

Create a BAC Library

Sampling of BAC clones

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BAC Fingerprinting

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What are the advantages of obtaining a complete inventory of plant coding sequence?

Detailed questions that may be approached through a genomics approach include:

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Arabidopsis contains 41 described gene families

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The tomato and potato genomes are very similar

Tomato-pepper co-linearity is dispersed

1-2% of plant genomes are composed of R gene clusters